Melilotate Hydroxylase

Kinetic and mechanistic studies on the oxidation of the melilotate hydroxylase . 2-OH-cinnamate complex by molecular oxygen.

The reaction mechanism of melilotate hydroxylase in the presence of 2-OH-cinnamate has been investigated by equilibrium titration, steady state kinetic and rapid reaction kinetic techniques. Analysis of the rapid reaction results for the reoxidation of reduced enzyme . 2-OH-cinnamate complex by molecular oxygen revealed the presence of an intermediate with visible absorption spectrum very simil...

Active-site probes of flavoproteins.

The chemical reactivity of 8-chloroflavins and 8-mercaptoflavins has been exploited in order to examine the orientation of protein-bound flavins relative to solvent. The apoprotein form of a series of flavoproteins was prepared and the native flavin was replaced by either 8-C1-flavin or 8-mercaptoflavin (FAD, FMN, or riboflavin form as was appropriate). The reconstituted proteins were exposed t...

Phenylalanine Hydroxylase, Tyrosine Hydroxylase, and Tryptophan Hydroxylase

Lysyl hydroxylase

1 1 4 7 jci.org Volume 125 Number 3 March 2015 Introduction Collagen is the most abundant intrinsic matrix scaffolding protein and contributes to tissue tensile strength (1). Various aspects of collagen metabolism, including its expression, deposition, organization, and turnover, are aberrant in epithelial cancer and have been implicated in tumor progression (2). Evidence from multiple types of...

Mouse phenylalanine hydroxylase. Homology and divergence from human phenylalanine hydroxylase.

The laboratory mouse represents an important model for the study of phenylalanine metabolism and the pathochemistry of phenylketonuria, yet mouse phenylalanine hydroxylase (PAH) has not been extensively studied. We report the cloning and sequencing of a mouse PAH cDNA, the expression of enzymic activity from the mouse PAH cDNA clone and the identification of mouse PAH and human PAH by two-dimen...